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武汉赛培新增文献：大鼠超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙 二醛（MDA）、肿瘤坏死因子（TNF）- α、白 细 胞 介 素IL-6及IL-1β ELISA试剂盒
发布时间：2021-10-19 18:15:18
【摘要】 目的 探讨七叶皂苷钠对大鼠脑缺血再灌注损伤的作用及机制。方法 60只成年SD大鼠随机分为假手术组、模型组 和七叶皂苷钠组，各20只。采用线栓法建立大鼠局灶性大脑中动脉闭塞再灌注损伤模型，七叶皂苷钠组建模成功后1 h腹腔注 射七叶皂苷钠（2.8 mg/kg），假手术组和模型组腹腔注射等量生理盐水。造模后24 h，使用Longa评分评估大鼠神经功能损伤程 度，使用称重法检测大鼠脑水肿程度，使用ELISA法测定大鼠损伤脑组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛 （MDA）、肿瘤坏死因子（TNF-α）、白细胞介素6（IL-6）及白细胞介素1β（IL-1β）的含量，使用TUNEL法检测损伤脑组织神经元 凋亡率，使用蛋白免疫印迹法检测损伤脑组织双特异性磷酸酶1（DUSP1）、核转录因子-κB（NF-κB）p65、Bcl-2及Bax蛋白的表 达。结果 与假手术组比较，模型组大鼠Longa评分显著增加（P<0.01），脑含水量显著升高（P<0.01），损伤脑组织MDA、TNF-α、 IL-1β和IL-6水平显著增高（P<0.001），损伤脑组织SOD和CAT水平显著降低（P<0.001），损伤脑组织神经元凋亡率显著升高 （P<0.01），损伤脑组织DUSP1和Bcl-2蛋白表达水平显著降低（P<0.05），损伤脑组织NF-kB p65和Bax蛋白表达水平显著增高 （P<0.05）。七叶皂苷钠显著逆转大鼠上述反应（P<0.05）。结论 七叶皂苷钠显著改善大鼠脑缺血再灌注损伤，机制可能与减轻 炎症反应、氧化应激反应、神经元凋亡等有关。
【关键词】 脑缺血再灌注损伤；七叶皂苷钠；炎症反应；氧化应激反应；细胞凋亡
【文章编号】 1009-153X（2021）08-0616-06 【文献标志码】 A 【中国图书资料分类号】 R 743
Protective effect of sodium aescinate on adult rats after cerebral ischemia-reperfusion injury WANG Lei, HU Huo- jun, MA Jin- yang, HUANG Song, DONG Yuan- xun, WANG Xu- guang, ZHOU You- dong. Institute of Neurology, China Three Gorges University; Department of Neurosurgery, Yichang Central People's Hospital, Yichang 441021, China 【Abstract】 Objective To explore the effect of sodium aescinate on the adult rats after cerebral ischemia- reperfusion injury (CIRI). Methods Sixty adult SD rats were randomly divided into sham operation group, model group and aescinate group, with 20 rats in each group. A rat model of focal middle cerebral artery occlusion and reperfusion injury was established by the modified Zea- Longa method. The aescinate group was intraperitoneally injected with sodium aescinate (2.8 mg/kg) 1 hour after the injury, and the sham operation and model groups were intraperitoneally injected with saline. Twenty-four hours after the injury, the Longa score was used to evaluate the neurological damage, the weighing method was used to detect the degree of brain edema, the ELISA method was used to detect the levels of superoxide dismutase (SOD) and catalase (CAT), malondialdehyde (MDA), tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in the injured brain tissues, the TUNEL method was used to detect the neuronal apoptosis rate in the injured brain tissues, and the Western blotting was used to detect the expression levels of dual-specific phosphatase 1 (DUSP1), nuclear transcription factor-κB (NF-κB) p65, Bcl-2 and Bax in the damaged brain tissues. Results after CIRI, the Longa score was significantly increased (P<0.01), the brain water content, the neuronal apoptosis rate, the levels of MDA, TNF- α, IL-1β, IL-6 NF- kB p65 and Bax were significantly increased in the injured cerebral tissues (P<0.001), and the levels of SOD, CAT, DUSP1 and Bcl- 2 were significantly reduced in the injured cerebral tissues (P<0.001). Sodium aescinate significantly reversed the above reactions in the CIRI rats (P<0.05). Conclusions Sodium aescinate significantly improves the CIRI in adult rats, and the mechanism may be related to reducing inflammation, oxidative stress, and neuronal apoptosis. 【Key words】 Cerebral ischemia-reperfusion injury; Aescinate; Adult rat; Inflammation; Oxidative stress; Neuronal apoptosis
