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新增赛培产品文献：小鼠细胞因子 IL-2、IL-10、TGF-β1 ELISA试剂盒
发布时间：2021-10-19 17:50:57
[摘要] 目的 研究龙葵碱对荷肝癌小鼠体内调节性 T 细胞（Treg 细胞）介导肝癌细胞免疫逃逸的影响及其作用机 制。 方法 建立肝癌 H22 细胞移植瘤小鼠模型，并设对照组、龙葵碱组、TGF-β1 抑制剂组和龙葵碱联合 TGF-β1 抑制剂组。 14 d 处理称取各组小鼠移植瘤质量，ELISA 法检测各组小鼠外周血中 IL-2、IL-10、TGF-β1 等免疫性 抑制细胞因子的含量变化，流式细胞术检测各组小鼠脾淋巴细胞培养液中 CD4+、CD4+ CD25+ Foxp3+ Treg 的表达水 平，免疫荧光法检测小鼠脾淋巴细胞培养液中 Foxp3+ 免疫荧光强度。 结果 14 d 后，与对照组相比，龙葵碱组、 TGF-β1 抑制剂组和联合组的肝癌荷瘤小鼠肿瘤瘤重量均显著低于对照组，差异有统计学意义（P<0.05）；龙葵碱 组、TGF-β1 抑制剂组、联合组 3 组小鼠外周血中免疫性抑制细胞因子 IL-2、IL-10、TGF-β1 均有明显降低（P<0.05）；龙 葵碱组、TGF-β1 抑制剂组、联合组三组 CD4+表达水平均高于对照组，CD4+ CD25+ Treg 的比例则明显下降（P<0.05）； 免疫荧光染色结果显示，龙葵碱组、TGF-β1 抑制剂组、联合组 3 组荷瘤小鼠 Treg 细胞中 Foxp3+的表达明显低于 对照组，差异有统计学意义（P<0.05）。 结论 龙葵碱可能通过降低荷瘤小鼠 Treg 细胞含量来改善免疫逃逸，从而 发挥抗肿瘤作用。
[关键词] 龙葵碱；肝癌；免疫逃逸；Treg 细胞
[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-9701（2021）18-0035-04
Impacts of solanine on tumor immune escape mediated by Treg cells in hepatocellular carcinoma GAO Juwei XU Kai RAN Ran YING Yinyin Department of Oncology, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310012, China [Abstract] Objective To research the impacts of solanine on hepatoma cells immune escape mediated by regulatory T cells (Treg cells) in hepatocarcinoma bearing mice and its action mechanism. Methods The mice model of H22 cell transplantation tumor was established and they were divided into the control group, the solanine group, the transforming growth factor-β1 (TGF-β1) inhibitor group and the solanine combined with TGF-β1 inhibitor group. After 14 days of treatment, the weight of transplanted tumor in each group was measured, and the level changes of immune inhibitory cytokines such as IL-2, IL-10 and TGF-β1 in the peripheral blood of mice in each group were detected by the enzyme linked immunosorbent assay (ELISA) method. The expression levels of CD4+ , CD4+ CD25+ Foxp3+ Treg in spleen lymphocyte culture medium of mice in each group were detected by the flow cytometry method, and the immunofluorescence intensity of Foxp3 + in spleen lymphocyte culture medium of mice was detected by the immunofluorescence method. Results After 14 days, compared with the control group, the tumor weights of the hepatocarcinoma bearing mice in the solanine group, the TGF-β1 inhibitor group and the combined group were all significantly lower than that in the control group, with statistically significant differences (P< 0.05). The levels of immunosuppressive cytokines such as IL-2, IL-10 and TGF-β1 in the peripheral blood of mice in the solanine group, the TGF-β1 inhibitor group and the combined group were all significantly decreased (P<0.05). The expression levels of CD4 + in the solanine group, the TGF-β1 inhibitor group and the combined group were higher than those in the control group, while the proportion of CD4+ CD25+ Treg decreased significantly (P<0.05). Immunofluorescence staining showed that the expressions of Foxp3 + in Treg cells of hepatocarcinoma bearing mice in the solanine group, the TGF-β1 inhibitor group and the combined group were significantly lower than those in the control group (P<0.05). Conclusion Solanine may play an anti-tumor role by reducing the content of Treg cells in hepatocarcinoma bearing mice to improve immune escape. [Key words] Solanine; Hepatocellular carcinoma; Immune escape; Treg cells
龙葵碱对肝癌Treg细胞介导的肿瘤免疫逃逸的影响_高聚伟.pdf
